This article will go over complex ideas from repair pathways to Cas 9 variants. These are the things you need to understand if you want to dive deep into this area. — In this article, I will go in-depth about many technical concepts in CRISPR-mediated genome editing. Table of Contents DNA, RNA, Amino Acids, Peptides, Enzymes;
Gene regulation, transcription, plasmids, translation;
Double Stranded Breaks, Single Stranded Breaks;
Deletions, Insertions, Substitutions;
Non-homologous end joining; homology directed repair;
The CRISPR mediation genome editing;
Prime editing;
Base editing;
CRISPRa/i;
Cas 9 Variants: SpCas9, SaCas9, eSpCas9, HypaCas9, xCas9…